Department of Chemistry Office: (310) 338-2943
and Biochemistry Home: (310) 641-8030
Loyola Marymount University
Los Angeles, CA 90045-8225

EDUCATION

Degree Institution

Ph.D. (Chemistry) University of California
July, 1984 Berkeley, CA

A.B. (Chemistry/Classics) Williams College
June, 1977 Williamstown, MA

RECENT RESEARCH

Studies on the Structure and Function of Familial Amytrophic Lateral Sclerosis "Muteins" of Cuprozinc Superoxide Dismutase (Loyola Marymount University, 1994-present)

Differential Scanning Calorimetry of myohemerythrin, its apoprotein, and metal-substituted derivatives. (Loyola Marymount University, 1991-1994)

Kinetic and Spectroscopic studies of mutants of cytochrome c peroxidase. (Post-doctoral fellowship, Department of Molecular Biology, Scripps Clinic and Research Foundation, La Jolla, CA 92037: 1989-91)

Physical studies of the interaction between metal ions and bovine cuprozinc superoxide dismutase, including calorimetric and metal ion-affinity measurements. (Post-doctoral fellowship, Department of Chemistry and Biochemistry, UCLA 1985-89)

TEACHING EXPERIENCE

Associate Professor of Chemistry and Biochemistry, LMU (1997)

Assistant professor of Chemistry and Biochemistry, LMU (1991-1997)

Adjunct assistant professor, lecturing freshman chemistry.
(UCLA 1987 & 1988)

MEMBERSHIPS

Phi Beta Kappa; Sigma Xi; Am. Chem. Soc.; The Protein Society; AAAS.

RECENT PUBLICATIONS

"Chemistry" in The Volume Library (2000 ed), Rollie J. Myers, Jr. and James A. Roe, Vol 1, pp. 475-508,Southwestern Press, Nashville.

"Mutations in copper-zinc superoxide dismutase that cause amyotrophic lateral sclerosis alter the zinc binding site and the redox behavior of the protein," Lyons, Thomas J.; Liu, Hongbin; Goto, Joy J.; Nersissian, Aram; Roe, James A. ; Graden, Janet A.; Cafe, Carla; Ellerby, Lisa M.; Bredesen, Dale E.; et al.Proc. Natl. Acad. Sci., USA (1996) 93, 12240-12244.

"Altered Reactivity of Superoxide Dismutase in Familial Amyotrophic Lateral Sclerosis." Wiedau-Pazos, Martina; Goto, Joy J.; Rabizadeh, Shahrooz; Gralla, Edith B.;Roe, James A.; Lee, Michael K.; Valentine, Joan S.; Bredesen, Dale E.Science (1996) 271, 515-518.

"Unusual Trigonal-Planar Copper Configuration Revealed in the Atomic Structure of Yeast Copper-Zinc Superoxide Dismutase." Ogihara, Nancy L.; Parge, Hans E.; Hart, P. John; Weiss, Manfred S.;Goto, Joy; Crane, Brian R.; Tsang, Joyce; Slater, Kelly;Roe, James A.; et al. Biochemistry (1996) 35, 2316-2321.

"New Type 2 Copper-Cysteinate-Proteins. Copper Site Histidine-to-Cysteine Mutants of Yeast Copper-Zinc Superoxide Dismutase." Lu, Yi; Roe, James A. ; Bender, Christopher J.; Peisach, Jack; Banci, Lucia; Bertini, Ivano; Gralla, Edith B.; Valentine, Joan Selverstone Inorganic Chemistry (1996) 35, 1692-1700.

"Role of the Bridging Histidyl Imidazolate Ligand in Yeast Copper-Zinc Superoxide Dismutase." Journal of Inorganic Chemistry 116 (1994): pp. 9743-9744

"Metalloprotein Ligand Redesign: Characterization of the Copper-Cysteinate Proteins Derived from Yeast Copper-Zinc Superoxide Dismutase," Lu, Y.; Roe, J.A., Gralla, E.B.; Valentine, J.S. in "Bioinorganic Chemistry of Copper," Karlin, K.D. & Tyeklar, Z., eds., pp. 64-77 (Chapman & Hall, New York, 1993).

"Thrombin-Binding DNA Aptamer Forms a Unimolecular Quadruplex Structure in Solution," Macaya, R. F.; Schultze, Smith, F.W.; Roe, J.A.; Feigon, J. Proc. Natl. Acad. Sci USA 90, 3745-3749 (1993).

"Construction of a 'Blue' Copper site at the Native Zinc Site of Yeast Copper-Zinc Superoxide Dismutase," Lu, Y.; LaCroix, L.B.; Lowery, M.D.; Solomon, E.I.; Bender, C.J.; Peisach, J.; Roe, J.A.; Gralla, E.B.; Valentine J.S. J. Am. Chem. Soc. 115, 5907-5918 (1993).

"Enhanced Oxidation of Aniline Derivatives by Two Mutants of Cytochrome c Peroxidase at Tryptophan 51," Roe, J.A.; Goodin, D.G. J. Biol. Chem. 268, 20037-20045 (1993).

"The Redesign of a Type II Copper Protein into a Type I: Construction and Characterization of Yeast Cu,Zn Superoxide Mutants," Lu, Y.; Gralla, E.B.; Roe, J.A.; and J.S. Valentine. J. Am. Chem. Soc. 114, 3560-3562 (1992).

"Amino Acid Substitutions at TRP-51 of Cytochrome c Peroxidase: Effects on Coordination, Species Preference for Cytochrome c, and Electron Transfer," Goodin, D.B.; Davidson, M.G.; Roe, J.A.; Mauk, G.A.; and M. Smith., Biochemistry 30, 4953-4962 (1991).

"Complexometric Titration of Protein Bound Metal Ions: A Method for Determining Binding Constants," Roe, J. A.; and J. S. Valentine, Anal. Biochem. 186, 31-40 (1990).

"Silver Binding Properties of Bovine Cuprozinc Superoxide Dismutase," Roe, J. A.; Peoples, R.; Scholler, D.; and J. S. Valentine, J. Am. Chem. Soc. 112, 1538-1545 (1990).

"Differential Scanning Calorimetry of Cu,Zn-Superoxide Dismutase, the Apoprotein, and Its Zinc-Substituted Derivatives," Roe, J. A.; Butler, A.; Scholler, D. M.; Valentine, J. S.; Marky, L.; and K. J. Breslauer. Biochemistry 27, 950-958 (1988).

"Magnetic and Spectroscopic Characterization of an Iron Porphyrin Peroxide Complex. Peroxoferrioctaethylporphyrin (1-)," Burstyn, J. N.; Roe, J. A.; Mikstal, A. R.; Shaevitz, B. A.; Lang, G.; and J. S. Valentine. J. Am. Chem. Soc. 110, 1382-1388 (1988).

"Studies of Copper-Zinc Superoxide Dismutase: The Role of Zinc and of the Essential Arginyl Residue," Valentine, J. S.; Roe, J.A.; Butler, A.; and D. Mota de Freitas, in Biological & Inorganic Copper Chemistry, vol. I, Eds., Karlin, K. D. and J. Zubieta, pp. 53-60, Adenine, New York (1985).

Presentations

"Investigation of the Thermal Stability of Streptomyces seoulensis NiSOD," Niemoth-Anderson JD, Rodriguez JA, Lee J-W, Roe JA, YimY-I, Diane E. Cabelli DE, Valentine JS, Sa-Ouk Kang S-O, and Maroney MJ, National Meeting of the American Chemical Society held in San Francisco, March 26-30

"Purification, Metal Ion Content, and Thermodynamic Stability of 14 Mutant Superoxide Dismutases Associated with Familial ALS." Hayward LJ, Rodriguez J, Jang G, Cabelli DE, Roe JA, Valentine JS, and Brown RH Jr . The 11th Intl Symposium on ALS/Motor Neuron disease, 4-6 Dec, Arhus,
Denmark.

"The Binding of Silver Ions to the Active Site of Cu,Zn-Superoxide Dismutase," Roe, J. A.; Peoples, R.; Scholler, D.; and J. S. Valentine. Pacific Conference on Chemistry and Spectroscopy, Irvine, CA (October 1987).

"Kinetic Aspects of the Differential Scanning Calorimetry of Cu,Zn-Superoxide Dismutase," Roe, J. A.. San Francisco State University (October 1986) and Jackson State University (November 1986).

"Differential Scanning Calorimetry of Cu,Zn-Superoxide Dismutase, the Apoprotein, and Its Zinc-Substituted Derivatives," Roe, J. A.; Butler, A.; Scholler, D. M.; Valentine, J. S.; Marky, L.; and K. Breslauer. The 40th Calorimetry Conference, Asilomar (August 1985).

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