Samuel Lane has an estimated birth date of 1800 in Chowan County, North Carolina. He was the son of Samuel Lane. He married Rhody Tomlinson, daughter of Thomas Tomlinson and Mary 'Polly' Rountree, in North Carolina. Samuel was recorded on the census of 1 June 1850 living in South Creek District, Beaufort County, North Carolina. He was enumerated as Samuel Lane, the head of household, age 50. The other members of his household were William Lane age 14, Mary Lane age 10 and John Lane age 5. His will was dated 3 April 1859, probate June Term 1859. His death was estimated to be 1859 in South Creek, Beaufort County, North Carolina.