December 10, 2006
Three years since this page was last updated ... I moved from CHLA to
City of Hope, and now on to Sacramento.
Multi-Probe Microscopy (http://home.earthlink.net/~mpmicro/mpmicro.zip) is
over 1500 pages.
PubSpectra is online (
http://home.earthlink.net/~pubspectra/ ) and Carl Boswell and I
published a Cytometry article about
it and Carl's spectral graphing website (see
Another year gone by since my last update...
Multi-Probe Microscopy (http://home.earthlink.net/~mpmicro/mpmicro.zip) is almost
to 900 pages.
I have been a co-author on three peer reviewed papers: Chantrain et al,
Xang et al and Xang et al. Please see my home page for details or check out
my Biography page.
I am collaborating with Dr. Car Boswell, U. Arizona, on the next
generation of his spectral traces for fluorescent dyes web site (sorry, not
online yet). Click
here for Carl's original web site. I had nothing to do with his original
site, except to email him a "well done" comment. For version 2, I'm
collecting the spectral data, Carl and his UA colleagues are handling the
database, web server/web interface design.
I also thought it would be nice to add Tiki Goddess to this page. She is
shown below (scroll down further for her 'mate', Tiki God or click
here) or click on her icon in the left hand navigator bar click
here for a full page image of Tiki Goddess.
Been an eventful spring and summer. I'm writing this on Monday October 28,
the morning after daylight losing time ended (or started?). Nice to drive to
work in daylight, though I suspect driving home in darkness will not be so
Let's see... at work we upgraded our Leica DMRA to a Leica DMRXA; we've
started to use our Leica TCS SP confocal microscope a lot more (if you have
LCS 2.770 or 2.871, ask Leica for a newer version. We currently use 2.968 but
were told after the service person installed it that they stopped
distributing...should have a new version in November). Our local Leica rep,
Layla from McBain Instruments, recently demo'd the "gliding stage"
for our Leica MZ FL III stereomicroscope...$300 "no brainer"
purchase". Layla also briefly demo'd the new Leica stereo 2x lens (NA of
0.285, I believe). We also plan on purchasing the 2x lens. It will go on the
MZ's second lens mount -- we have the Meyer Instruments "on
axis" dual objective lens adapter. This clever gizmo lets us shift the
objective to under the right eye "lenslet" so that we can acquire
vertical Z-series. A second benefit is that it mounts 2 lenses. We currently
have the standard 1x and a 0.4x lens. I anticipate we will use the 1x and 2x,
but since the full range of the 1x is covered by the 0.4x + 2x, we might
eventually settle on that (have to buy the lens first). For image acquisition
on the MZ, we use Compix SimplePCI (5.0 beta, www.c-imaging.net)
with a Hamamatsu ORCA-ER scientific grade digital CCD camera. Performance and
price wise, SimplePCI kicked butt on Media Cybernetics Image Pro+ (4.0 or
4.5) and MetaMorph 4.0 and 4.5. SimplePCI is also the first imaging software
to support the MZ focus drive.
We've been having a lot of error messages with our Polaroid SprintScan
4000 when using our Meyer Instruments Pathscan Enabler. The SprintScan was
$2000 when we bough it two years ago. It has been superceded by the 4000Plus
which is now $1200 (and Polaroid got out of the scanner business as part of
their attempt to recover from bankruptcy). The SprintScan repair cost is
guesstimated at $400-600, so when a Polaroid service rep mentioned that an
alternative was to upgrade (trade-in) to the 4000Plus for $650, another
no-brainer (especially since the 4000Plus uses the professional grade
SilverFast Ai). FYI - the SprintScan 4000 uses the same insides as the
Microtek ArtixScan 4000TF, and both can use the PathScan Enabler. ...
Speaking of the PathScan, Christophe Chantrain, Yves DeClerck and I have an
article in press at J. Histochem. Cytochem. on quantitative
immunohistochemistry with the PathScan (now published: Chantrain
et al 2003). One of my favorite Pathscan images is our
Image Core mascot: Tiki God (see below or click
here for a full page version of Tiki God or scroll up or click here for Tiki
Please email me (firstname.lastname@example.org)
if you have trouble figuring out the parts of our Tiki God. A couple of
hints: the bright red feature is a heart, the nasty grin is liver. On
second thought, considering that I've never taken an anatomy class, you would
probably be better off asking someone who has.
If you've had trouble finding my temporal area map (TAM) write-up, click
here for information on TAM and TAM
My window was fixed mid-May 2002. My office then felt stuffy!
Busy, busy, busy. Too busy to do much with this web site. I was pretty
good about updating our intranet site (//shelia/image
for my colleagues at CHLA), until May 2001 when my server died for a month.
After that I was busy trying to get a confocal like gizmo called the OptiGrid
working. That took until January 2002 to get resolved (we sent it back
because it turned out not to be suitable for our multi-user environment --
your mileage may be better .. or try Zeiss's Apotome). Then I finally got back to the intranet
site. On March 11, 2002, 9:30 am, something smashed my office window as
shown below (I suspect a terrorist pigeon, since it seemed a bit early in the
day for an L.A. drive by shooting and my office is on the 10th floor).
Made a nice stained glass effect until the wind picked up and the glass
fell out (and in). Fortunately I'm in L.A., not MN, WI, MI, or even PA, and
the window fasts east, away from the occasional rainstorm (yes, L.A. does get
rain). Talk about distractions!
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