1.

Underline the coding regions in pencil and place the abbreviations for the amino acids above the proper codons.

2.

Highlight the CAT box in yellow.

3.

Highlight the TATA box in green.

4.

Circle the start codon.

5.

Draw a box around the stop codon.

6.

Underline all BamHI sites in blue.

7.

Underline all EcoRI sites in red.

8.

Underline all HindIII sites in green.

9.

Which restriction enzyme is the best choice to excise this gene for transformation into a bacteria vector?

10.

Find the exons for the following gene:
PHE-PHE-LEU-MET-ALA-GLU-GLY-HIS-TYR-ARG-CYS-TRP-SER-PHE-
GLY-PHE-LEU-PRO-ALA-GLY-ARG-THR-GLU-TYR-CYS-PRO-THR-
ARG-SER-ASN

11.

List the one-letter abbreviations for the amino acids given in question 11 in proper sequence.
__  __  __  __  __  __  __  __  __  __  __  __  __  __  __
__  __  __  __  __  __  __  __  __  __  __  __  __  __  __