From the end-on junction with a former L&N line at Chattahoochee (MP 842.5), where an ACL line once left on the north side and the Apalachicola Northern starts and runs parallel, to the east, on the south side, the former SAL single track line, in Yard Limits, with overall speed limit 49 mph (because this is "dark territory"), heads northeast, past a road crossing at Main Street, east-southeast, past MP 838.6, where the AN line turns away south, Yard Limits end and DTC starts (the Gretna Block), and the line turns east, past MP 834.9, where the freight speed limit falls to 45 mph, a turn south-southeast, and MP 833.7, where full line speeds resume, Mt. Pleasant, a detector at MP 830.8, the 2,146 ft. north side siding at Gretna (MP 828.7), a curve to the east, and the 8,200 ft. south side siding at Douglas City (MP 825.6), where the Gretna Block ends (MP 824.9) and the Quincy Block starts.
Speed limits fall to 35/25 at MP 823.7, where the line starts a tight clockwise turn to the west of due south, past Quincy, where a spur from the Quincy Mine trails in on the east side, MP 823.0, where the freight speed limit rises to 35 mph, MP 822.8, where the passenger speed limit rises to 40 mph, a turn curvily east and then one curvily south-southeast, past MP 818.5, where the freight speed limit rises to 40 mph, MP 818.2, where speed limits rise to 49/45, MP 817.0, where the Quincy Block ends and the Tallahassee Block starts, a turn southeast, then south, and then curvily east, past the 2,621 ft. south side siding at Midway (MP 811.4), a turn southeast past a detector at MP 809.8 and Ochlocknee, where it turns south-southeast, past MP 805.7, where the freight speed limit falls to 40 mph, and a turn east past MP 805.3, where the freight speed limit rises to 45 mph.
A turn southeast again leads to MP 802.9, where the Tallahassee Block ends, CTC starts, the former Georgia, Florida & Alabama line trails in from the north, and the passenger speed limit falls to 45 mph (but overall line speeds rise to 79 mph for passenger trains, 60 mph for intermodal trains, and 50 mph for freight trains, because signals are provided east of this location), and the line turns east, past Tallahassee Yard (Dale Mabry Field), north where the GF&A and two SAL lines once trailed in from the south and the GF&A once left to the north-northwest, past a road crossing at Lipona Road (MP 801.3), MP 800.4, where speed limits fall to 35/20 and the line turns east where a connector from the GF&A once trailed in from the west, MP 799.8, where passenger speeds also fall to 20 mph, the Tallahassee passenger station, MP 799.4, where passenger speeds rise to 30 mph, and the 1,710 ft. south side siding at Tallahassee (MP 799.3).
The only urban area along this portion of the route is Tallahassee, the state capital and site of a large university.