Define the following terms:
1. enzyme:
2. amylase:
3. enzyme activity:
List 4 different characteristic variables that can cause a change in, but not totally destroy enzyme activity. Indicate whether a large increase in each factor would cause an increase (+) or a decrease (-) in enzyme action.
...(+/-)
1. ____ ____________________________________________________________________________
2. ____ ____________________________________________________________________________
3. ____ ____________________________________________________________________________
4. ____ ____________________________________________________________________________
Prepare graphs of your experimental results on graph paper:
GRAPH #A: Standard curve (Beer's Law) - Absorbence verses maltose concentration. Plot each point relating absorbence to volume of maltose solution for test tubes A1-A5.
Draw a straight line through these points, NOT from point to point. (see the graph on the following page). This graph is called a standard curve, and will be used by the other groups as a graphical calculator to convert from absorbance to µl of maltose.
