Genetic Engineering Toolbox




Description and Procedure:

Specially modified nucleotides used in the Sanger Method. The 3' carbon of dideoxy-nuclotides lack a hydroxyl group which prevents the addition of the next nucleotide thus terminating further polymerization.

Uses or Function:

They are used to stop the construction of the complementary strand of DNA. Using all four dideoxy-nucleotides in 4 separate test tubes followed by fragment separation (Sanger Method), one can sequence a gene or fragment of DNA.