Genetic Engineering Toolbox

Dideoxy-nucleotides


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Description and Procedure:

Specially modified nucleotides used in the Sanger Method. The 3' carbon of dideoxy-nuclotides lack a hydroxyl group which prevents the addition of the next nucleotide thus terminating further polymerization.


Uses or Function:

They are used to stop the construction of the complementary strand of DNA. Using all four dideoxy-nucleotides in 4 separate test tubes followed by fragment separation (Sanger Method), one can sequence a gene or fragment of DNA.


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