Genetic Engineering Toolbox



Viruses and bacterial plasmids

Description and Procedure:

Insertion of foreign DNA into a host (plasmid) is accomplished by adding restriction enzymes which cleave both vector and foreign DNAs at the same restriction site. Since the sticky ends are the same, some foreign DNA will be annealed to the vector's DNA. Lygase is used to bond them together. Plasmids and viruses can be easily replicated in their host bacteria.

Uses or Function:

Recombination across species barrier. Gene replacement therapy.