Genetic Engineering Web Quest


Researchers can work with the DNA from one organism and incorporate it into the cell of a different species.

Sometimes this occurs natrually but the process is random and generally the vector involved in such a transfer is an unwanted virus.

Follow the links below to find out how researchers can purposefully engineer new capabilities for bacteria, domestic crops, and even humans.


In this web quest you will discover how and why bacteria are used to make a human protein like insulin.

Bacteria are often the organisms of choice for molecular cloining. List three advantages for why prokaryotes such as bacteria are used as host for cloning. Scan down this page to find the answer.

1) ______________________________________________________________

2) ______________________________________________________________

3) ______________________________________________________________

What are some of the disadvantages posed by the use of bacteria?

1) ______________________________________________________________

 

2) ______________________________________________________________

 

 

The first step is the creation of a "library" of genes from the species we need a protein product from. Since an individual gene is needed but only chromosomes or mRNA from entire cells are available researchers must use

What is a DNA or genome library? ________________________________________________

How does the genome library differ from a cDNA library? _____________________________

 

A cDNA library uses mRNA. How can the mRNA be converted back to the DNA representing a gene?

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What role did retroviruses play in providing the molecular tool to convert mRNA back to DNA?

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